Structure and function of alternative and multi-stranded DNAs, RNAs, and nucleic acid/protein complexes: Novel microarrays production for characterization of intact genomic factors
As a scientist I perform molecular biological and histotechnological research on the structure and function of conventional (e.g., right-handed B-DNA), alternative (e.g., Z-DNA) and multi-stranded (e.g., triplex DNA) DNA and RNA molecules, in both normal and diseased tissue [e.g., eye (ocular lens) (cataracts), and skin (epidermis) (e.g., melanoma, Xeroderma Pigmentosum)].
As a nucleic acid nanotechnologist, I develop the next generation of DNA and RNA microarrays [i.e., Novel multistranded and alternative DNA, RNA and plasmid microarrays (transitional structural nucleic acid microarrays)]. This involves new ways of immobilizing intact, non-denatured unaltered nucleic acids to enhanced substrate surfaces. In addition I am also involved in developing techniques that can be used with the novel microarrays, viz., original genomic and proteomic techniques/methods (i.e., transitional structural chemogenomics, transitional structural chemoproteomics, transitional structural pharmacogenomics, transitional structural pharmacoproteomics).
Furthermore, I develop novel approaches to fixing tissues for superior histotechnological processing of preserved DNA, RNA and nucleic acid-protein complexes in tissues. These tissues can then be better characterized immunohistochemically and histochemically for the presence of nucleic acids. Presently, I am trying to manufacture the novel microarrays using standard industry equipment and locating companies interested in licensing the Gagna/NYIT patent (i.e., Method for immobilizing multistranded nucleic acid molecules by modifying more than one strand thereof, and binding each strand to a solid support: August 30, 2005 - 6,936,461).
Gagna CE, Chan NJ , Farnsworth PN, Kuo H-R, Kanthala TR, Patel AH, Patel N, Law A, Patel PP, Richards SA, Yam T, Nici A, Lambert WC. (2009) Localization and quantification of intact, undamaged right-handed double-stranded B-DNA, and denatured single-stranded DNA in normal human epidermis and its effects on apoptosis and terminal differentiation (denucleation). Archives of Dermatological Research. 301:659-672.
Gagna CE, Lambert WC. (2009) Novel Multistranded, Alternative, Plasmid and Helical Transitional DNA and RNA Microarrays: Implications for Therapeutics. Pharmacogenomics. 10: 895-914.
Lambert WC, Gagna CE, Lambert MW (2008) Xeroderma Pigmentosum: Its Overlap with Trichothiodystrophy, Cockayne Syndrome and Other Progeroid Syndromes. Advances in Experimental Medicine and Biology. 637: 128-37.
Gagna CE, Kuo HR, Mitacek EJ, et al., (2007) Novel DNA Staining Method and Processing Technique for the Quantification of Undamaged Double-Stranded DNA in Epidermal Tissue Sections by PicoGreen Probe Staining and Microspectrophotometry. Journal of Histochemistry and Cytochemistry. 55: 999-1014